In Vitro-In Vivo Extrapolation: Fish Bioconcentration Factor (IVIVE – Fish BCF) Model

The OECD has developed standardized test guidelines for measuring in vitro biotransformation rates using S9 and hepatocyte (HEP) assay systems from rainbow trout liver tissues. Models are required to extrapolate the in vitro rates to in vivo liver clearance and whole body biotransformation rates and to incorporate these rates in toxicokinetic models to calculate bioaccumulation assessment endpoints such as the bioconcentration factor (BCF). One model for extrapolating in vitro S9 and HEP biotransformation rates to fish BCFs is the one developed by Nichols et al. 2013. An on-line version of the Nichols et al., 2013 IVIVE-Fish BCF model is available here.

– Nichols, J. W.; Huggett, D. B.; Arnot, J. A.; Fitzsimmons, P. N.; Cowan-Ellsberry, C. E. 2013. Toward improved models for predicting bioconcentration of well-metabolized compounds by rainbow trout using measured rates of in vitro intrinsic clearance. Environ. Toxicol. Chem. 32, (7), 1611-22. DOI: 10.1002/etc.2219